CSRP1 Promotes Colon Adenocarcinoma Growth and Serves as an Independent Risk Biomarker for Worse Prognosis.

Background: Cysteine and Glycine Rich Protein 1 (CSRP1) belongs to the cysteine-rich protein family, which contains a unique double-zinc finger motif and is important for development and cellular differentiation. Abnormal expression of CSRP1 was reported within several malignancies such as prostate cancer and acute myeloid leukemia. Here, we explored function of CSRP1 within colon adenocarcinoma (COAD) for the first time. Methods: The mRNA levels of CSRP1 in COADs were obtained from TCGA datasets. CSRP1 protein expressions in COADs were tested via immunohistochemistry staining. Patients' prognosis was evaluated using both univariate analysis and multivariate analysis. Two human COAD originated cancer cell lines, Caco-2, and HT-29, were used for cellular experiments including shRNA knockdown, proliferation assay, and migration assay. In vivo model was established using nude mice xenografts to further validate the role of CSRP1 in COAD progression. Results: The mRNA levels of CSRP1 are elevated in COAD specimens from patients with more advanced tumor stages and higher Carcinoembryonic Antigen (CEA) levels. In addition, higher CSRP1 mRNA level indicates worse COAD prognosis. Consistently, higher CSRP1 protein expression is correlated with worse overall survival according to both univariate and multivariate analysis, indicating that CSRP1 is a new COAD prognostic factor. Furthermore, COAD cells transfected with CSRP1-shRNAs exhibit attenuated proliferation and migration capacities. Finally, growth of xenografts originated from CSRP1-knockdown cells is inhibited comparing to the control ones. Conclusions: Expression of CSRP1 is positively correlated with COAD progression, which can promote tumor growth and migration. Higher CSRP1 can is a novel independent prognostic factor of COAD.

Survival benefit after neoadjuvant or adjuvant radiotherapy for stage II-III gastroesophageal junction adenocarcinoma: A large population-based cohort study.

Objective: The standard treatment for stage II-III gastroesophageal junction adenocarcinoma (GEJA) remains controversial, and the role of radiotherapy (RT) in stage II-III GEJA is unclear. Herein, we aimed to evaluate the prognosis of different RT sequences and identify potential candidates to undergo neoadjuvant RT (NART) or adjuvant RT (ART). Materials and methods: In total, we enrolled 3,492 patients with resectable stage II-III GEJA from the Surveillance, Epidemiology, and End Results (SEER) database, subsequently assigned to three categories: T1-2N+, T3-4N-, and T3-4N+. Survival curves were evaluated using the Kaplan-Meier method along with the log-rank test. We compared survival curves for NART, ART, and non-RT in the three categories. To further determine histological types impacting RT-associated survival, we proposed new categories by combining the tumor, node, and metastasis (TNM) stage with Lauren's classification. Results: ART afforded a significant survival benefit in patients with T1-2N+ and T3-4N+ tumors. In addition, NART conferred a survival advantage in patients with T3-4N+ and T3-4 exhibiting the intestinal type. Notably, ART and NART were both valuable in patients with T3-4N+, although no significant differences between treatment regimens were noted. Conclusions: Both NART and ART can prolong the survival of patients with stage II-III GEJA. Nevertheless, the selection of NART or ART requires a concrete analysis based on the patient's condition.

Preparation, characterization and food packaging application of nano ZnO@Xylan/quaternized xylan/polyvinyl alcohol composite films.

Xylan could be considered as a good potential candidate for food packaging film because of the vast source and biodegradability, however, its application was restricted by the drawbacks of poor film-forming property, humidity sensitivity, weak mechanical strength and poor antibacterial property. In this paper, xylan was firstly modified by quaternization to improve the film-forming property, then ZnO nanoparticles encapsulated by xylan (nano ZnO@Xylan) was prepared by nanoprecipitation method, finally a series of biodegradable composite films were prepared using quaternized xylan and polyvinyl alcohol with incorporation of nano ZnO@Xylan. The surface morphology, molecular structure and crystallography structure of the films were characterized. The addition of nano ZnO@Xylan decreased water vapor permeability and solubility, meanwhile obviously increased the ultraviolet shielding performance as well as the antibacterial properties of the films. The bacteriostasis rate of the films against E. coli and S. aureus reached up to 99 %. Furthermore, the preservation time of cherry tomatoes covered with ZnO@Xylan/QX/PVA films was extended to at least 21 days. In conclusion, all the results ensure that the fabricated composite films have considerable promising application in the food packaging industry.

miR-548d-3p inhibits the invasion and migration of gastric cancer cells by targeting GKN1.

BACKGROUND: The aim of this study was to explore the function and mechanism of GKN1 in gastric cancer (GC) progression. METHODS: Firstly, we used GEO2R to perform differential gene analysis on GSE26942 and GSE79973 and constructed the protein-protein interaction network of differential genes by STRING. Next, the cytoHubba, Mcode plugins, and GEPIA were used to obtain our follow-up research object GKN1. Then, the function of GKN1 in GC was verified by scratch and transwell assay in GC cells. We further analyzed the genes related to GKN1 through LinkedOmics, and exported top 100 genes positively or negatively correlated with GKN1. Meanwhile, Metascape was performed on these genes. Finally, we analyzed the miRNAs that bind to GKN1 through the miRDB and verified the correlation between miR-548d-3p and GKN1 using dual-fluorescence and quantitative PCR experiments. RESULTS: Bioinformatics analysis showed that there were 52 differential genes on GSE26942 and GSE79973. In addition, the results of functional assays indicated that overexpressed GKN1 can inhibit GC cell migration and invasion, while GKN1 knockdown demonstrated the opposite effect. Additionally, Metascape analysis results showed that the 3'-UTR region of mRNA is rich in AU sequences, based on which we infer that mRNA may be regulated by miRNA. Dual-fluorescence and quantitative PCR assays clarified that miR-548d-3p may be one of the target miRNAs of GKN1, which was up-regulated in GC tissues. CONCLUSIONS: In summary, we clarified that miR-548d-3p regulates GKN1 to participate in GC cell migration and invasion, and provides a possible target for the prognostic diagnosis and treatment of GC.